Auxin Requirements of Sycamore Cells in Suspension Culture

Auxin requirements of sycamore cells in suspension culture.

Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this ;auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic ac...

The structure of sycamore callus cells during division in a partially synchronized suspension culture.

Sycamore suspension callus cells have been partially synchronized to give a culture with a rmtotic index of 15 %. Living dividing cells of the culture have been examined with Nomarski differential interference optics and a comparable study made on fixed cells with the electron microscope. An organized band of reticulate cytoplasm partially encircles the nucleus at mitosis. The cell divides by t...

Carrot Suspension Culture Cells

Glycoprotein of the wall of sycamore tissue-culture cells.

1. A glycoprotein containing a large amount of hydroxyproline is present in the cell walls of sycamore callus cells. This protein is insoluble and remained in the alpha-cellulose when a mild separation procedure was used to obtain the polysaccharide fractions of the wall. The glycoprotein contained a high proportion of arabinose and galactose. 2. Soluble glycopeptides were prepared from the alp...

Growth of 293 cells in suspension culture.

A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.